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immunohistochemistry testing, immunohistochemistry staining, ihc staining, ihc controls

Quantitative IHControls® — Menu Overview

IHControls® are grouped together so that a single control will be useful for multiple different primary antibodies, often to different protein biomarkers. The groups are organized by subject area.  For example, HER2, ER, and PR are grouped together as a breast cancer panel. In addition to the different analyte specificities, there are also multiple different concentrations of each that are often available.  This ensures that your lab uses the correct concentration that is most sensitive.  Our studies have demonstrated that controls with too high a concentration are insensitive (1). There is an approximately ten-fold difference in antigen concentration between each level. We recommend using the lowest antigen concentration that provides an easily detectable stain. That concentration may not be the same for HER-2, ER, and PR and should be determined in your lab.

 

(1) K Vani, SR Sompuram, AK Schaedle, A Balasubramanian, M Pilichowska, S Naber, JD Goldsmith, KG Chang, F Noubary, & SA Bogen. The importance of epitope density in selecting a sensitive positive IHC control.  J. Histochem. Cytochem. 2017 65(8):463-477.

immunohistochemistry testing, immunohistochemistry staining, ihc staining, ihc controls

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BRLS11* – Antigen-high multi-analyte IHControls for HER2/ER/PR, incorporating controls for primary antibodies that may require the highest analyte concentration: HER2 – CB11 & 4B5, ER – EP1, PR – 16 & 636.

 

BRLUA04*– Antigen-intermediate multi-analyte IHControls.  The microbeads included in this container are immunoreactive with the following antibodies: ER – 1D5, SP1,6F11, 2-123, and EP1; PR – 636, 16, 1294, 1E2; HER-2 -Herceptest, CB11, 4B5, SP3.

 

BRLWA08*– Antigen-low multi-analyte IHControls The microbeads included in this container are immunoreactive with the following antibodies: ER – 1D5, SP1,6F11, 2-123, and EP1; PR – 636, 16, 1294, 1E2; HER-2 – Herceptest, CB11, 4B5, SP3.

 

BRLXA09*– Antigen-very low multi-analyte IHControls. The microbeads included in this container are immunoreactive with the following antibodies: ER – 1D5, SP1,6F11, 2-123, and EP1; PR – 636, 16, 1294, 1E2; HER-2 – Herceptest, CB11, 4B5, SP3.

 

*For research use only. Not for use in diagnostic procedures.

 

Coming Soon

 

Ki-67, for monoclonal antibodies MIB-1, 30-9, and SP6.

p53, for monoclonal antibody DO7, B53-11, B53-12

ALK, for monoclonal antibodies ALK1, ALK01, D5F3, OTI1A4,andSP8

Bcl-2, for monoclonal antibodies 124, 100/D5, 100

MLH1, for monoclonal antibodies ES05, M1, G168-15

MSH2, for monoclonal antibodies G219-1129, FE11, 25D12

MSH6, for monoclonal antibodies 44, EP49, EPR3945, PU29, SP93

PMS2, for monoclonal antibodies EPR3947, EP51, A16-4G