Scoring

immunohistochemistry testing, ihc controls, ihc testing

Better visibility

Evaluation and scoring of controls and calibrators are important in identifying potential immunostain problems. Unlike tissue sections, the analyte-coated microbeads in IHControls® and IHCalibrators stain comparatively homogeneously and do not require special training in histology for evaluation. Stain quality can easily be evaluated by histotechnologists.

 

 

immunohistochemistry calibrators, IHC calibrators

Strong staining

Strong staining

 

Rim staining + : The majority of microbeads have accentuated rim staining. Compare the rim staining to the rims of unstained microbeads to appreciate the accentuation.

 

Central staining + : The majority of microbead centers are stained.

 

Helpful tip: The strongly stained microbeads are (on average) of equal or greater color intensity to the smaller color standard microbeads.

immunohistochemistry calibrators, IHC calibrators

Moderate staining

Rim staining +, but weaker: Rim staining is present, but less intensely. Rim staining can be appreciated by comparing to unstained microbeads.

 

Central staining +/– : The centers of many microbeads are tinted brown, but often not uniformly throughout the microbead interior.

 

Helpful tip: The moderately stained microbeads are of a mildly-moderately weaker intensity than the small color control microbeads.

immunohistochemistry calibrators, IHC calibrators

Weak staining

Rim staining +/-: is barely detectable, which distinguishes stained microbeads from unstained beads.

 

Central staining –: The microbead interior appears washed out, without appreciable staining.

 

Helpful tip: The weakly stained microbeads are distinguishable from unstained microbeads, but the difference is subtle.