---
title: "Module 10. Summary: Understanding Metric Calibration"
description: "- Calibration is designed to improve consistency and comparability of IHC results across laboratories or across instruments within a laboratory. - The Metric Calibration Workflow steps include: Stain; Scan, Analyze, and Assess. - The Metric Calibration Report calculates and illustrates…"
url: "https://bostoncellstandards.com/learning-center/documentation/metric-calibration-tutorial/summary-understanding-metric-calibration/"
updated: "2026-09-01"
category: Metric Calibration Tutorial
---

# Module 10. Summary: Understanding Metric Calibration

- Calibration is designed to improve consistency and comparability of IHC results across laboratories or across instruments within a laboratory.
- The Metric Calibration Workflow steps include: Stain; Scan, Analyze, and Assess.
- The Metric Calibration Report calculates and illustrates key parameters of your assay, including LOD.
- LOD quantifies assay analytic sensitivity, which affects tissue stain intensity. Tissue stain intensity is responsible for the majority of IHC staining errors.
- Using the Metric Calibration Report, you can compare the sensitivity of your lab’s LOD with those of peer labs.