---
title: Module 4. Understanding Metric Calibration Report
description: "Once you have uploaded Calibrator slide images to Metric, the IHCalculations software automatically: - Analyzes the images and quantifies the stain intensity of the images. - Produces a Metric Calibration Report. Figure 4 below provides an example of a Metric…"
url: "https://bostoncellstandards.com/learning-center/documentation/metric-calibration-tutorial/understanding-metric-calibration-report/"
updated: "2026-09-01"
category: Metric Calibration Tutorial
---

# Module 4. Understanding Metric Calibration Report

Once you have uploaded Calibrator slide images to Metric, the IHCalculations software automatically:

- Analyzes the images and quantifies the stain intensity of the images.
- Produces a Metric Calibration Report.

Figure 4 below provides an example of a Metric Calibration Report. We have provided annotations (Text box and A, B, and C below) to explain the blue dots, the red fitted analytic response curve, and the calculation of LOD, which in this case is is 6960 molecules/cell equivalent.

 ![](https://bostoncellstandards.com/wp-content/uploads/2026/09/metriccalibrationreport_assay-8-10.svg)

*Figure 4*