Products

Setting the
Standard

Boston Cell Standards’ IHCalibrators™ are traceable to:

National Institute of Standards & Technology (NIST)

Standard Reference Material® 1934

Product Page-Link1@2x
Product Page-Link2@2x

Boston Cell Standards’ Solutions

Boston Cell Standard Product Photo-2@2x

IHControls®

IHControls® are on-slide IHC staining controls that use proprietary tagged peptide or protein targets. This technology provides for analytic traceability to an international concentration standard 1. This assures consistency day-to-day, lot-to-lot, and lab-to-lab and confirms that an assay worked properly. The performance of IHControls® in checking primary antibodies, detection kits, and antigen retrieval has been validated and published 2-4.

Boston Cell Standard Product Photo 9@2x

IHCalibrators™

IHCalibrators™ are similar in construction but provide for precise measurement of the limit of detection, the most important parameter of analytic sensitivity. With IHCalibrators™, clinical IHC laboratory staff can ensure that their immunostain is configured correctly, capable of sensitively detecting the cellular biomarker in question.

Product Benefits

Improved test accuracy
and reproducibility

Proactive early-warning of
assay deviations

Simplified, quantitative
regulatory compliance

Rapid, exact troubleshooting
when needed

Direct quantitative readout for
optimizing reagent concentrations

Ensures standardization among
multiple instruments

IHControls® are ½ -¼th the true
cost of homemade controls

Purchasing on-slide controls in a
ready-to-use format is
convenient; simpler workflow.

IHControls® have been extensively
tested and validated, as described
in a series of published papers

These advancements place the
clinical IHC lab on par with other
types of clinical diagnostic labs
which already have commercial
standardized test controls.

Learn how they work, and why they’re important

IHControls®

Multiplexed. IHControls® are grouped together so that a single control will be useful for multiple different primary antibodies, often to different protein biomarkers. The groups are usually organized by analyte or subject area. For example, HER2, ER, and PR are grouped together as a breast cancer panel.

Matched. In addition to the different analyte specificities, there are also multiple different concentrations of each that are often available. This ensures that your lab uses a concentration matched to your stain. Our studies have demonstrated that controls with too high a concentration are insensitive 1. There is an approximately ten-fold difference in antigen concentration between each level. We recommend using the lowest antigen concentration that provides an easily detectable stain. That concentration may not be the same for HER-2, ER, and PR and should be determined in your lab.

IHControls® Product Menu

Marker Description Level Target Antibodies Product Code
Breast Cancer Panel ER/PR/HER2 multi-controls High Optimal: ER EP1, PR 636, PR 16, HER2 CB11, HER2 4B5
Also binds: HER2 polyclonal
BRLS11
Breast Cancer Panel ER/PR/HER2 multi controls Medium Optimal: PR 1294, ER 1D5, ER 6F11, PR 1E2, HER2 SP3, HER2 polyclonal
Also binds: ER SP1, Targets of BRLS11 (high)
BRL2U04
Breast Cancer Panel ER/PR/HER2 multi controls Low Optimal: ER SP1
Also binds: Targets of BRL2U04
BRL2W08
PD-L1 PD-L1 controls High Optimal: SP142, ZR3
Also binds: SP263, 28-8, 22C3, E1L3N, 73-10
PDLH
PD-L1 PD-L1 controls Medium Optimal: SP263, 22C3, 28-8, ZR3, 73-10, E1L3N
Also binds: SP142
PDLM
PD-L1 PD-L1 controls Low Optimal:
Also binds: SP263, 22C3, 28-8, ZR3, 73-10, E1L3N
PDLL
Alk Alk controls High 5A4, D5F, SP8 AlkLH
Ki67 Ki67 controls High 30-9, MIB1 K67LH
P53 P53 controls High DO-7, P53-11, P53-12 p53LH

*IHControls® levels are intended to be matched to the customer’s IHC test. Optimal levels based on company experience is shown but should be verified in customer’s lab

**IHControls® are designed to serve as positive controls for assays testing for the human form of the above-described markers

IHCalibrators™

IHCalibrators™ are provided as a series of up to 10 dots, already positioned on a microscope slide, ready for use. Each of the calibrators has a different pre-measured concentration ranging from approximately 1,000–1,000,000 molecules per microbead. The concentrations are traceable to a NIST standard reference material. Our current product menu is as follows:

IHCalibrators™ Product Menu

Marker Intended Use Target Antibodies Product Code
Alk Alk calibrators D5F, ALK1, OTI1​ ALKD5R
Alk Alk calibrators SP8 ALKSP8R
bcl2 Bcl2 calibrators​ 100/20, 124​ bclR
CD20 CD20 calibrators​ L26 CD20R
ER SP1​ ER SP1 calibrators​ ER SP1​ BRRSP1​
ER EP1 ER EP1 calibrators​ ER EP1​ BRREP1​
ER 6F11​ ER 6F11 calibrators​ ER 6F11​ BRR6F​
HER2 C-terminus HER2 calibrators​ HER2 CB11, HER2 4B5, HER2 polyclonal​ BRRCB​
HER2 SP3​ HER2 SP3 calibrators​ HER2 SP3​ BRRSP3​
Ki67​ Ki67 calibrators​ 30-9, MIB1​ Ki67R​
MLH1 MLH1 calibrators​ ES05​ ES5R​
MSH2 MSH2 calibrators​ G219​ G219R​
MSH6​ MSH6 calibrators​ EP49​ EP49R​
MSH6​ MSH6 calibrators​ SP93 SP93R
NTRK​ NTRK calibrators​ EPR17341​ NT41R​
PD-L1 intracellular​ PD-L1 calibrators​ E1L3N, ZR3, 73-10, SP142, SP263​ PDR​
PD-L1 extracellular​ PD-L1 calibrators​ 22C3, 28-8​ PDEXR​
PMS2 PMS2 calibrators​ EP51, EPR3947​ EP51R​
PR 1E2​ PR 1E2 calibrators​ PR 1E2, PR 1294​ BRR1E2​
PR 636​ PR 636 calibrators​ PR 636​ BRR636​
PR 16​ PR 16 calibrators​ PR 16​ BRR16​
p53​ p53 calibrators​ DO7, BP53-11​ p53R​
p63​ p63 calibrators​ 4A4​ p63R​
ROS1 ROS1 calibrator​ ROS1D4D6​ ROSR​
H. pylori H Pylori calibrators​ (In development) H. pylori polyclonal HPR​
Human IgG​ Human IgG protein calibrators​ Human IgG​ hIgG​
Uromodulin Uromodulin calibrators​ Uromodulin rb polyclonal​ UDR​
CD3​ CD3 calibrators​ CD3 Rb polyclonal​ CD3R​
Aquaporin 1 Aquaporin calibrators​ Aquaporin 1/22​ AQPR
Myeloperoxidase MPO calibrators​ Rb polyclonal​ MPOR​

*IHCalibrators™ are designed to serve as positive controls for assays testing for the human form of the above-described markers.

Purchase products now