BCS IHCalibrators® make Calibration possible for IHC
- 15 small round spots pre-printed on a microscope slide
- The spots are glass microbeads covalently coated with a fluorescent biomarker
- Each bead has a precise concentration of the biomarker traceable to a primary standard at NIST
- The numbers provide an orientation to the sequence of increasing biomarker concentrations
- A certificate of analysis provides the exact concentrations for each level
- Identify the lowest concentration level that is still stained.
- In each Panel, level 5 is the lowest that still has sufficient color intensity to consider positive.
- Look at the Certificate of Analysis for that calibrator and correlate it with the actual concentration.
- The actual Lower Limit of Detection (LOD) is likely at a concentration between levels 4 and 5.
- Metric calculates the precise LOD.
- IHCalibrators® enable this graph
- Metric calculates LOD and dynamic range and establishes control ranges
- Select an IHControl® within the dynamic range
- Compare your LOD with other peer labs
